Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a

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Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a.

Chlorophyll b is an ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. Its biosynthesis plays a key role in the adaptation to various light environments. We isolated six chlorophyll b-less mutants by insertional mutagenesis by using the nitrate reductase or argininosuccinate lyase genes as tags and examined the rearrangement of mutant genomes. We found that an overla...

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The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana.

Chlorophyll b is synthesized from chlorophyll a and is found in the light-harvesting complexes of prochlorophytes, green algae, and both nonvascular and vascular plants. We have used conserved motifs from the chlorophyll a oxygenase (CAO) gene from Chlamydomonas reinhardtii to isolate a homologue from Arabidopsis thaliana. This gene, AtCAO, is mutated in both leaky and null chlorina1 alleles, a...

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Modulation of Chlorophyll b Biosynthesis and Photosynthesis by Overexpression of Chlorophyllide a Oxygenase (CAO) in Tobacco

Chlorophyll (Chl) b is synthesized by oxidation of a methyl group on the B ring of the porphyrin molecule to a formyl group by chlorophyllide (Chlide) a oxygenase (CAO). The overexpression of Arabidopsis thaliana full length CAO (AtCAO) in tobacco (Nicotiana tabacum) resulted in an increased Chl synthesis and a decreased Chl a/b ratio in low-light-grown (LL) as well as in high-light-grown (HL) ...

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Chlorophyll a + b Content and Chlorophyll Fluorescence in Avocado

Tonnage (T), Simmonds (S) avocado trees, and TxS crosses were evaluated for differences in chlorophyll content and maximal quantum yield of photosystem II in sun and shade-type leaves. Total chlorophyll content by area (Chl a+bar) ranged from 984 mg m in TxS240 to 4320 mg m in Simmonds. Chlorophyll a/b ratio (Chl a/b) ranged from 9.8 to 5.5 in TxS238 and TxS243, respectively. Tonnage and Simmon...

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Chlorophyll b to chlorophyll a conversion precedes chlorophyll degradation in Hordeum vulgare L.

This study reveals by in vivo deuterium labeling that in higher plants chlorophyll (Chl) b is converted to Chl a before degradation. For this purpose, de-greening of excised green primary leaves of barley (Hordeum vulgare) was induced by permanent darkness in the presence of heavy water (80 atom % (2)H). The resulting Chl a catabolite in the plant extract was subjected to chemical degradation b...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1998

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.95.21.12719